A symmetry or asymmetry: Functional and compositional comparison of venom from the left and right glands of the Indochinese spitting cobra (Naja siamensis).

Contralaterally positioned maxillary (upper jaw) venom glands in snakes are mechanically independent, being able to discharge venom from either gland separately. This has led some studies to test venom function and composition of each contralaterally positioned venom gland to investigate any differences. However, the data on the subject to-date derives from limited sample sizes, appearing somewhat contradictory, and thus still remains inconclusive. Here, we tested samples obtained from the left and right venom glands of four N. siamensis specimens for their relative binding to the orthosteric site of amphibian, lizard, snake, bird, and rodent alpha-1 nicotinic acetylcholine receptors. We also show the relative proteomic patterns displayed by reversed phase liquid chromatography - mass spectrometry. Our results indicate that three of the venom gland sets showed no difference in both functional binding and composition, whilst one venom gland set showed a slight difference in functional binding (but not in specificity patterns between prey types) or venom composition. We hypothesise that these differences in functional binding may be due to one gland having previously ejected venom at some time prior to venom extraction, whilst its contralateral counterpart did not. This might cause the differential rate of toxin replenishment to be unequal between glands, thus instigating the difference in potency, likely due to uneven toxin proportions between glands at the time of venom extraction. These results demonstrate that the separate venom producing glands in snakes remain under the same genetic control elements and produce identical venom components.

A Taxon-Specific and High-Throughput Method for Measuring Ligand Binding to Nicotinic Acetylcholine Receptors.

The binding of compounds to nicotinic acetylcholine receptors is of great interest in biomedical research. However, progress in this area is hampered by the lack of a high-throughput, cost-effective, and taxonomically flexible platform. Current methods are low-throughput, consume large quantities of sample, or are taxonomically limited in which targets can be tested. We describe a novel assay which utilizes a label-free bio-layer interferometry technology, in combination with adapted mimotope peptides, in order to measure ligand binding to the orthosteric site of nicotinic acetylcholine receptor alpha-subunits of diverse organisms. We validated the method by testing the evolutionary patterns of a generalist feeding species (Acanthophis antarcticus), a fish specialist species (Aipysurus laevis), and a snake specialist species (Ophiophagus hannah) for comparative binding to the orthosteric site of fish, amphibian, lizard, snake, bird, marsupial, and rodent alpha-1 nicotinic acetylcholine receptors. Binding patterns corresponded with diet, with the Acanthophis antarcticus not showing bias towards any particular lineage, while Aipysurus laevis showed selectivity for fish, and Ophiophagus hannah a selectivity for snake. To validate the biodiscovery potential of this method, we screened Acanthophis antarcticus and Tropidolaemus wagleri venom for binding to human alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-9, and alpha-10. While A. antarcticus was broadly potent, T. wagleri showed very strong but selective binding, specifically to the alpha-1 target which would be evolutionarily selected for, as well as the alpha-5 target which is of major interest for drug design and development. Thus, we have shown that our novel method is broadly applicable for studies including evolutionary patterns of venom diversification, predicting potential neurotoxic effects in human envenomed patients, and searches for novel ligands of interest for laboratory tools and in drug design and development.

Deception detection, transmission, and modality in age and sex.

This study is the first to create and use spontaneous (i.e., unrehearsed) pro-social lies in an ecological setting. Creation of the stimuli involved 51 older adult and 44 college student "senders" who lied "authentically" in that their lies were spontaneous in the service of protecting a research assistant. In the main study, 77 older adult and 84 college raters attempted to detect lies in the older adult and college senders in three modalities: audio, visual, and audiovisual. Raters of both age groups were best at detecting lies in the audiovisual and worst in the visual modalities. Overall, college students were better detectors than older adults. There was an age-matching effect for college students but not for older adults. Older adult males were the hardest to detect. The older the adult was the worse the ability to detect deception.

Practitioners' views on elder mistreatment research priorities: recommendations from a Research-to-Practice Consensus conference.

This article presents recommendations from expert practitioners and researchers regarding future directions for research on elder abuse prevention. Using the Research-to-Practice Consensus Workshop model, participants critiqued academic research on the prevention of elder mistreatment and identified practice-based suggestions for a research agenda on this topic. The practitioners' critique resulted in 10 key recommendations for future research that include the following priority areas: defining elder abuse, providing researchers with access to victims and abusers, determining the best approaches in treating abusers, exploiting existing data sets, identifying risk factors, understanding the impact of cultural factors, improving program evaluation, establishing how cognitive impairment affects legal investigations, promoting studies of financial and medical forensics, and improving professional reporting and training. It is hoped that these recommendations will help guide future research in such a way as to make it more applicable to community practice.

Unwarranted Assumptions about Children's Testimonial Accuracy.

We examine eight unwarranted assumptions made by expert witnesses, forensic interviewers, and legal scholars about the reliability of children's eyewitness reports. The first four assumptions modify some central beliefs about the nature of suggestive interviews, age-related differences in resistance to suggestion, and thresholds necessary to produce tainted reports. The fifth unwarranted assumption involves the influence of both individual and interviewer factors in determining children's suggestibility. The sixth unwarranted assumption concerns the claim that suggested reports are detectable. The seventh unwarranted assumption concerns new findings about how children deny, disclose, and/or recant their abuse. Finally, we examine unwarranted statements about the value of science to the forensic arena. It is important not only for researchers but also expert witnesses and court-appointed psychologists to be aware of these unwarranted assumptions.

The perceived credibility of older adults as witnesses and its relation to ageism.

In experiment 1, 267 undergraduates read a case summary and witness statement. Sex and age of the witness (49, 69, 79 or 89 years) were varied. Participants rated the witness's perceived convincingness, confidence, quality of observation, accuracy, honesty, competence, memory, suggestibility, and cognitive functioning. As well as an age effect for honesty, age by sex interactions were observed for several characteristics, particularly for comparisons of the 79-year-olds versus the 49-year-olds, and 89-year-olds versus the 49-year-olds. In experiment 2, 94 undergraduates read the same testimony given by a 79-year-old male or female witness, and completed the Fraboni Scale of Ageism and the Aging Semantic Differential. Participants who evidenced stronger aging stereotypes on these measures rated the witness less favorably than did participants who were less prejudiced. This experiment is the first to show a link between perceived credibility of older adults and ageist attitudes. Practical applications regarding how older witnesses are viewed by jurors, and the criminal justice system more generally, are discussed.